The cataract is one of the leading causes of blindness in the world. The surgery is the only effective treatment to overcome the cataract and consists in removing the cloudy lens and replacing it by an artificial intraocular lens (IOL). Secondary cataract, or Posterior Capsular Opacification (PCO), is the most common postoperative complication of the cataract surgery. PCO is raised from the host response to the implant: the residual lens epithelial cells (LEC) proliferate, migrate, transdifferentiate to mesenchymal cells which form a cloudy layer and enclose the intraocular lens, causing patients to lose vision again. Following implantation of an IOL, the human host responses include protein adsorption, cell adhesion, inflammation, and wound healing. These foreign body reactions are all initiated from the first step of protein adsorption from aqueous humor or blood instantaneously after implantation of the IOL in the capsular bag. Numerous factors such as the chemical composition of the material, the design, the roughness, the bioadhesive character of the IOLs play a role in the appearance of these complications. In the present work, an optimized sample preparation protocol to identify and quantify the “adsorbomes” of hydrophilic and hydrophobic materials for IOLs known to have a higher or a lower incidence of PCO, respectively, was obtained. Polymer disks incubated in a physiological environment simulating aqueous humor (diluted human serum) were used. Quantitative analysis of common proteins in all samples was made after trypsin digestion on disks and peptide analyses by mass spectrometry (UPLC-SYNAPT G2, MSE-IMS acquisition). Albumin, immunoglobulins G, apolipoproteins and vitronectin are a first common protein signature of hydrophilic and hydrophobic acrylic materials. After quantification, it will be possible to correlate the relative intensities within protein signature and the surface properties, as well as low or high cell adhesion percentages of (LEC) on polymers.