Additive inhibitory effect of combined peptides with ATPase inhibitor, membrane permeabilizing agent, or detergent against Burkholderia pseudomallei (#119)
Siritongsuk Pawinee
1
2
,
Patramanon Rina
1
2
,
Madhongsa Kanjana
1
2
- Protein and proteomics research affiliation, Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
- Department of Biochemistry, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
Burkholderia pseudomallei (BP) is the causative agent of melioidosis, the endemic disease causing septic shock in Southeast Asia and northern Australia. BP is intrinsically resistant to conventional antibiotics, resulting in difficulty in treatment. Antimicrobial peptides (AMPs) are alternative to classical antibiotics and does not cause resistance due to different mode of action. Combination effect between AMPs and other agents have been proved to increase the bacterial inhibitory effect. This study aimed to determine antimicrobial activity of AMPs alone and combination of AMPs with ATPase-inhibiting agent (NaN3) or detergent (TritonX-100 and Tris) on live BP. The selected peptides were bactenecin (RLCRIVVIRVCR-NH2), BMAP-18 (GRWKRWRKKWKKLWKKLS-NH2), CA-MA (KWKLFKKIGKFLHSAKKF-NH2), and RTA3 (RPAFRKAAFRVMRACV-NH2). When testing AMPs without combination, bactenecin exhibited strongest inhibition as seen in percentage of killing. When combining peptides with NaN3 or Tris, all combination exhibited stronger inhibitory effect than treating with peptides or the agents alone. However, the only CA-MA combining with TritonX-100 exhibited stronger effect that the peptides or the agent alone.