Actinopyga lecanora, a type of sea cucumber commonly known as stone fish with relatively high protein content, was used as raw material to generate bioactive peptides using six commercial proteases. Among the enzymes used, bromelain hydrolysate showed the multifunctional bioactivities including antimicrobial, antioxidant, ACE inhibitory and NO production inhibition. Therefore, the bromelain protein hydrolysate was fractionated based on the hydrophobicity and isoelectric point of their peptides content. The total, twelve peptides were identified using UPLC-MS/MS system, synthesized and their biological activities were evaluated. Among the peptides identified three novel bioactive peptides revealed multifunctional activities including antibacterial against Pseudomonas aeruginosa (48.64-65.5%), Pseudomonas sp. (40.26-51.32%), Escherichia coli (61.76%) and Staphylococcus aureus of (45.30-50%); antioxidative (68.4-93.3%); ACE inhibitory (59.9-98.12%); and ability to inhibit LPS-induced NO production in RAW 264.7 cells (56.32-69.94%).These multifunctionalbioactive peptides would be used in the formulation of various functional foods, and nutraceuticals.
*Corresponding author: Prof. Dr. Nazamid Saari, Department of Food Science, Faculty of Food Science and Technology, University putra Malaysia, 43400 upm, Serdang Selangor, Malaysia. Tel: o3-89468385, Fax: 03-89423552
E-mail: nazamid@putra.upm.edu.my