Poster Presentation 10th Australian Peptide Conference 2013

Global in-depth quantitative proteomic analysis of HIV infected cells using a novel Q-OT-qIT mass spectrometer (#207)

Lin Tang Goh 1 , S Eliuk 1 , J Johnson 1 , V Zabrouskov 1 , N Krogan 1
  1. Thermo Scientific, To be confirmed, tbc, Australia

Quantitative changes in the phosphoproteome of human cell lines following infection by the human immunodeficiency virus (HIV) were analysed by hybrid Orbitrap-based mass spectrometry. Global identification and quantification of changes in human phosphoproteome in response to HIV was performed using stable isotope labeled (SILAC) Jurkat cells and HILIC/TiO2 fractionation and enrichments using the new Orbitrap FusionTM TribridTM mass spectrometer and EASY-ETDTM source. More phosphopeptides were identified using a combination of ETD and CID fragmentation in a single run (via novel data dependent decision tree with dynamic scan management) than when using CID fragmentation alone which permitted precise localization of phosphorylation more frequently.